The Typhoid IgG/IgM Test is a qualitative membrane strip based immunoassay for the detection of Typhoid antibodies (IgG and IgM) in Serum / Plasma. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgM anti-Typhoid, if present in the specimen, will bind to the Typhoid conjugates. The immunocomplex is then captured by the reagent pre-coated on the T2 band, forming a burgundy colored T2 band, indicating a Typhoid IgM positive test result and suggesting a fresh infection. IgG anti-Typhoid if present in the specimen will bind to the Typhoid conjugates. The immunocomplex is then captured by the reagent coated on the T1 band, forming a burgundy colored T1 band, indicating a Typhoid IgG positive test result and suggesting a recent or repeat infection. Absence of any T bands (T1 and T2) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the T bands. Otherwise, the test result is invalid and the specimen must be retested with another device
Materials and Components
1.Specimen collection container
Allow the test device and specimens to equilibrate to room temperature (15-30℃ or 59-86℉) prior to testing.
Place the test device on a clean and levelsurface. 3. Hold the dropper vertically and transfer 2 drops of specimen (approximately 80μl) to the specimen well(S) of the test device, then add 1 drop of buffer (approximately 40μl) and start the timer.
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